FIGURE 5.

Effects of AGR2 expression on EGFR and other EGFR ligands. RNA quantification by qPCR of EGF, TGFα, HBEGF, and EGFR in H460 (A) and JH-EsoAd1 (B) cells with (WT) and without (KD) reduced AGR2 expression. C, AREG, EGF, TGFα, HBEGF, and EGFR mRNA levels after AGR2 overexpression in OE33 cells. BTC, EPREG, and EPNG were not detectable and are not shown. Control OE33 cells were transfected with pcDNA3.1-GFP. The insets contain an expanded image for EGF, TGFα, HBEGF, and EGFR. All values in the figure were normalized to β-actin. Error bars represent ± 1 S. D. (n = 3).