FIGURE 7.

AGR2 overexpression results in AKT phosphorylation via enhanced AREG expression. Blocking antibodies were used to determine whether AREG mediated the AGR2-induced AKT phosphorylation in OE33 cells. Two different concentrations of anti-AREG antibodies were applied to OE33 cells with (AGR2) or without (vector) AGR2 overexpression. Protein immunoblotting was then performed on the resultant cell lysates with antisera for either phosphorylated or total AKT. Densitometry was performed of the immunoblots and depicted on the graph as the pAKT/total AKT ratio.