FIGURE 8.

AREG mediates the AGR2 induced transformed phenotype. A, cell proliferation assay of H460 cells AGR2WT, AGR2KD, and AGR2KD_AREG that were cultured in 24-well plates (1 × 10⁴ cells/well) in 0.5 ml of DMEM supplemented with 0.5% FBS. The cells were harvested at the specified time points and manually counted. Each data point represents the mean of three wells; error bars = ± 1 S. D. B, assay of anchorage-independent growth of the same cells as above. Cells are plated in soft agar at different initial densities and assessed for colony number after 2 weeks. Column height represents the mean colony number from three different dishes; error bars, ±1 S.D.