Fig. 7.
Pnck promotes transition from the S to G2 phase of cell cycle. A: release from thymidine synchronization. Cells were plated at low density in complete medium and allowed to recover for 48 h. Cells were then synchronized by treatment with 5 mM thymidine for 48 h, after which they were washed twice in complete medium and released in complete medium for the indicated times. Cells were trypsinized, fixed in 75% ethanol, DNA stained with propidium iodide, and analyzed by flow cytometry. B: release from nocodazole synchronization. Cells were processed as in A above, except that synchronization was performed by treatment with 50 ng/ml nocodazole for 16 h.