Fig. 6.

Biochemical properties of membrane fractions isolated from Ryr1^+/+ and Ryr1^D/D mice. A: maximal specific binding capacity (B_max) values of [³H]ryanodine binding to crude skeletal muscle membrane fractions of 1.5- to 5.5-mo-old Ryr1^+/+ and Ryr1^D/D mice were determined as described in experimental procedures. Normalized data are means ± SE of 4–6 experiments. *P < 0.05 compared with Ryr1^+/+ mice. B: ⁴⁵Ca²⁺ uptake rates by crude membrane fractions of 1.5- to 5.5-mo-old Ryr1^+/+ and Ryr1^D/D mice were determined as described in experimental procedures. Normalized data are means ± SE of 4–5 experiments.