Fig. 5.

Wild-type and mutant Cx50 hemichannels have similar single-channel properties. A and B: single-channel current traces for wild-type Cx50 (A) and G46V (B) in the cell-attached mode. Voltage-clamp steps of ± 40 mV were applied from a holding potential of 0 mV. C and D: open channel current-voltage (I-V) curves for wild-type Cx50 (C) and G46V (D) obtained by application of a 200-ms voltage ramp between −100 mV and +100 mV to cell-attached patches containing only one active channel. Voltage-clamp ramp data were corrected for leakage currents as described in materials and methods.