Fig. 7.

Coexpression of G46V mutant with wild-type lens connexins decreases oocyte viability. Graphs represent the number of surviving oocytes in media containing 1 mM Ca²⁺ at different times following injection of connexin cRNA. A: oocytes were injected with Cx50, G46V, or a mixture of equal amounts of Cx50 and G46V (Cx50 + G46V) cRNAs, or no cRNA (AS) and incubated for 1, 24, 48, or 72 h at 18°C. The total amount of cRNA injected was 6.6 ng/oocyte. B: oocytes were injected with wild-type Cx46, G46V, or a mixture of equal amounts of wild-type Cx46 and G46V (Cx46 + G46V) cRNAs, or no cRNA (AS) and incubated for 1, 24, 48, or 72 h at 18°C. The total amount of cRNA injected was 6.9 ng/oocyte, except in the case of Cx50 + G46V where it was 13.8 ng/oocyte. The number of oocytes injected with each type of cRNA is indicated in parentheses.