Fig. 9.

G46V has a dominant behavior when mixed with wild-type Cx50. A: representative family of current traces recorded from single oocytes coinjected with cRNA for G46V and wild-type Cx50 in NaCl solution containing 0.7 mM [Ca²⁺]_o and 1 mM [Mg²⁺]_o. Voltage-clamp steps were applied to voltages between −80 mV and +30 mV in 10-mV increments from a holding potential of −80 mV. No leakage current correction was applied. Dashed line represents zero current. B: tail I-V relationships for G46V (■, n = 5), Cx50 (◊, n = 1), or G46V + Cx50 (1:1, ○, n = 4). Initial amplitudes of tail currents were measured at −80 mV after 20-s pulses to different potentials and plotted as a function of pulse potential. The total amount of cRNA injected was 0.147 ng/oocyte, except in the case of Cx50 + G46V where it was 0.294 ng/oocyte.