Induction of apoptosis in PC cell lines treated with B-DIM, erlotinib (Erl), gemcitabine (Gem), and the combination, which was evaluated by the ELISA assay. Cells were treated with 25 μM B-DIM, 2 μM erlotinib, 10 nM gemcitabine, or the combination. There was a significant potentiation in the induction of apoptosis in BxPC-3, COLO-357, and HPAC cells treated with B-DIM in combination with erlotinib or gemcitabine and triple combination as compared to cells treated with either agent alone.