Fig. 1.

CR increases blood adiponectin levels in both LF and HF-fed C57BL/6 mice. HF, high fat; LF, low fat; AL, ad libitum fed; CR, calorie restricted; P, pair fed. Energy intake of LF-AL mice was calculated based on daily food intake. HF-P or HF-CR mice received HF diet with calories equal to those of LF-AL and LF-CR mice, respectively. Restriction of energy intake for LF-CR and HF-CR mice was gradually applied: 20% in week 1, 30% in week 2, and then 40% of the value for LF-AL mice. A: blood samples were collected at indicated time points and stored for analysis. Serum adiponectin was measured using Western blotting with an internal reference sample between gels. Relative adiponectin levels were quantified by scanning band densities using Quantity One software. B: body weights were measured every week. C: epididymal fat was collected and weighed at the end of the study after overnight fasting. Data are presented as mean ± SE. *P < 0.05, **P < 0.001, LF-CR or HF-CR vs. LF-AL;#P < 0.05 HF-AL or HF-P vs. LF-AL at the same time point; n = 10 per group.