Fig. 3.

CR increases adiponectin gene expression but has no effect on blood adiponectin clearance rate. After 24-wk feeding regimens in C57BL/6 mice, as described in Fig. 1, epididymal fat tissues were collected. Levels of adiponectin mRNA (A) and protein (B) were measured using real-time PCR or Western blot. C: ¹²⁵I-labeled adiponectin (0.1 μCi) was injected into mice through the tail vein. Blood samples were collected, and radioactivities of precipitated serum proteins were measured. Circulating adiponectin half-life was calculated. Data are presented as means ± SE. *P < 0.05, LF-CR, HF-CR, or HF-AL vs. LF-AL; n = 10.