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CR increases PPARα gene expression in WAT. Total RNA samples were prepared from epididymal fat of 24-wk feeding regimen-treated C57BL/6 mice. mRNA levels of indicated genes were measured using real-time PCR with a relative quantification setting (A, B, and D, n = 10). Protein levels of indicated genes were measured using Western blotting (C, n = 6). Data are presented as means ± SE. *P < 0.05 vs. LF-AL;#P < 0.01 vs. LF-AL.
