Fig. 1.
The Prader-Willi syndrome (PWS) homologous gene region in mouse is functional in the pancreas. A: genetic map and transgenic deletion mouse model of PWS (TgPWS) deletion in mouse chromosome 7C. Circles, protein-coding genes; ovals, RNA-coding genes or transcripts (Ipw and Ube3a-as have no known coding potential; not all copies of miR-344, Snord116, and Snord115 are shown); black circles and ovals, paternally expressed; gray circles, maternally expressed; white circles, biparentally expressed; IC, imprinting center; cen, centromere; tel, telomere. Gray bar indicates 6.8-Mb deletion in TgPWS mice. B: mRNA expression of imprinted, paternally expressed genes from the TgPWS deletion region in mouse pancreas and NIT-1 insulinoma cells. RT-PCR was used to assess mRNA expression, with insulin 2 (Ins2) and Polr2a used as controls. H2O controls had no RNA.