Fig. 4.

Comparison of the mRNA and protein expression levels of myosin heavy chain (MHC) in PLN muscle of the Ossabaw swine on control, MetS, and DMetS diets. mRNA expression of different MHC isoforms was evaluated using quantitative real-time PCR (QRT-PCR, A). Representative image of Western blots evaluating protein expression of MHC1 and MHC2, as well as total MHC (MHC1&2) is shown in B. Relative protein expression level of MHC1 and MHC 2 after normalization for the total MHC expression in each sample is shown in C. *Significant difference between control and MetS and DMetS diet groups according to the t-test. There was no significant difference between control and MetS and DMetS diet groups according to the ANOVA.