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. 2009 Apr 1;81(1):222–230. doi: 10.1095/biolreprod.108.074666

FIG. 2.

FIG. 2.

Effect of latrunculin B and jasplakinolide on actin dynamics in HuF cells. A) The densitometric evaluation of the G-actin:F-actin ratio (labeled as G-/F-actin ratio) in HuF cells pretreated with latrunculin B (Latr B; 1 μM) or jasplakinolide (Jas; 100 nM) and either harvested immediately after 1 h of treatment with the drugs (1 h; white bars) or collected after 1 h of treatment with the drugs followed by incubation with 2% SFBS-RPMI for 24 h (1 h/24 h; black bars). The G-actin:F-actin ratio in untreated cells (Ctr) was set as 1, and other treatments were compared with the control. The blot at the top shows representative immunoblots of the distribution of beta-actin (ACTB) in the G-actin and F-actin fractions of HuF cells detected after 1 h of treatment with the drugs (1 h; upper blot) and after 1 h of treatment with the drugs followed by incubation with 2% SFBS-RPMI for 24 h (1 h/24 h; lower blot). B) The densitometric evaluation of the G-actin:F-actin ratio (labeled as G-/F-actin ratio) in HuF cells pretreated for 1 h with jasplakinolide (Jas; black bars) or with vehicle control (Ctr; white bars), followed by the treatment for 6 days with hormones (H), IL1B and hormones (IL1B + H), cAMP and hormones (cAMP + H), or untreated controls (Ctr). The blot at the top shows representative immunoblots of the distribution of ACTB in the G-actin and F-actin fractions of HuF cells treated with jasplakinolide (Jas; lower blot) or without pretreatment (upper blot) as detected after 6 days of decidualization treatment of the cells. Note the overall decrease in the G-actin:F-actin ratio detected after 6 days of decidualization treatment in HuF cells pretreated with jasplakinolide compared with HuFs without pretreatment.