Fig. 3.

Lentiviral overexpression of constitutively active Akt1 in cells infected for 5 days does not increase the relative abundances of the smooth muscle (sm) contractile apparatus-associated proteins SM22 or sm-α-actin, calculated relative to those of β-actin in the same cultures. Top: typical Western blots from a single experiment. Bottom: mean ± SE SM22 or sm-α-actin abundances (relative to that of β-actin in the same cultures) in arbitrary units (P > 0.25 by uncorrected unpaired t-test, all comparisons). Similar results were obtained from serum-fed (n = 4) and serum-free (n = 3–4) cultures. myrAkt, cultures infected with pLenti6-myrAkt; LacZ, cultures infected with pLenti6-LacZ.