Fig. 3.

Mean ± SE 24-h intake of 0.51 M NaCl across Na appetite trials. A: experiment 1A: rats with bilateral ibotenic acid LHx (n = 23) ingested less NaCl than controls (n = 9) in the second furosemide (Furo) trial (*P ≤ 0.05), but they did not differ during the saline trial. During the first Furo trial, the NaCl intake of the LHx rats was similar to that during the second Furo and saline trials (P = 0.14; 0.43). Control rats consumed more NaCl during the second Furo trial than the first (P ≤ 0.0001), but their intake during both Furo trials was more than the saline trial (P ≤ 0.03; 0.0001). B: experiment 1B: rats with larger bilateral LHx (n = 18) ingested less NaCl than the controls (n = 7) during the Furo trial (*P ≤ 0.05) but not during the saline trial. Rats with LHx consumed equivalent volumes of NaCl during both the Furo and saline trials (P = 0.51). The controls, however, ingested more NaCl after the Furo injection than after saline (P = 0.01). C: experiment 2: rats with asymmetric lesions of the parabrachial nuclei and the LH (n = 13, Asym) ingested less NaCl than their controls (n = 10) on each of 3 Furo trials but not after the saline test (*P ≤ 0.05). The Asym group actually ingested equivalent amounts of NaCl on 3 Furo and the saline trials. The controls, however, consumed more NaCl during the second and third Furo trials than the first. The saline test was done between the second and third Furo trials, but the results are depicted last in the graph for clarity.