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. 2011 Mar 2;300(5):R1115–R1125. doi: 10.1152/ajpregu.00806.2010

Fig. 6.

Fig. 6.

A: schematic representation of the fluorescence-activated cell sorting (FACS) strategy. B: Dot plots showing FACS staining profiles and gating (black boxes) of adipose stromal vascular fraction. The live singlets were analyzed on the basis of lack of CD31 and Ter119 expression and were further separated on the basis of expression of CD45. CD45− cells (Lin−) were then analyzed on the basis of expression of CD34 and CD29, and the CD34+ cells were analyzed on the basis of staining for Sca-1 and CD24. C: representative dot plots for adipocyte progenitor cells (APC) by flow cytometry. D: analytic data for brown APC (Lin−:CD29+:CD34+:Sca-1+:CD24+). *P < 0.05 vs. SED-ND; †P < 0.05 vs. SED-HFD; n = 8.