Fig. 7.

Preadipocytes from brown adipose tissue were cultured and differentiated into adipocytes, which were examined by immunocytochemistry for uncoupling protein 1 (UCP1). Representative images (A and B) and statistical analysis (C) for immunohistochemistry of UCP1 in cells by in vitro study. Representative images (E and F) and statistical analysis (G) for immunohistochemistry of UCP1 in interscapular brown adipose tissue from mice fed HFD. *P < 0.05 vs. SED-HFD; **P < 0.001 vs. SED-HFD; n = 8. BAT, interscapular brown adipose tissue.