Fig. 3.

Immunoblotting for LC3-II. LC3-II specifically associates with autophagosomes and not with any other vesicular structures. The intensity of the LC3-II bands was increased in cpk mice compared with wild-type (+/+) mice (A). In +/+ mice treated with bafilomycin A1, there was an increase in LC3-II (A). In cpk mice treated with bafilomycin A1, LC3-II was not increased (A). LC3-II was increased in Cy/Cy rats compared with +/+ and Cy/+ rats (B) compared with respective normal littermate controls. β-Actin, used as a loading control, was not different among the groups. *P < 0.05 vs. controls; n = 4/group.