Fig. 2.

Vtn genotyping and protein immunolocalization after UUO. A: representative gel of genomic DNA analyzed by PCR illustrates the 442-bp wild-type (+/+) allele and the 280-bp mutant (−/−) allele. B: representative Western blot of kidney protein samples 14 days after UUO illustrates a distinct ≈70-kDa band in the Vtn+/+ mice and an absence of this band in the Vtn−/− mice. Immunofluorescence confocal photomicrographs illustrate interstitial Vtn accumulation in a Vtn+/+ kidney 14 days after UUO (C) and absence of the same protein in a Vtn−/− kidney (D). Magnification ×400.