Fig. 3.
Flow response of δ-α chimeras. A: schematic representation of the wild-type δ, δN-TM1-α, δECL-α, δTM2-C-α, and α-constructs. B: relative response to LSS of wild-type and δ-α chimeras. Experiments were performed with oocytes expressing δβγ, δECL-αβγ, δTM2-C-αβγ, and αNsiI-XhoIβγ (αβγ channels). The relative response represents the ratio of the peak benzamil-sensitive current following vertical perfusion to the basal benzamil-sensitive current (Ipeak/Ibasal). C: time constants (τ) of activation by LSS of wild-type channels and δ-α chimeras. ENaC currents were not observed in oocytes expressing δN-TM1-α channels. Statistically significant differences are indicated as *P < 0.05, **P < 0.01, and ***P < 0.001 (n = 15–29) by a Kruskal-Wallis test following by Dunn's multiple comparisons test.