Fig. 4.
TM2 segment determines the time course of activation by LSS. A: schematic representation of wild-type α-, δTM2-α, δC-α, αTM2-δ, αC-δ, and δ-constructs. B: relative response to LSS of wild-type channels and chimeras. Experiments were performed with oocytes expressing αNsiI-XhoIβγ (αβγ), δClaI-XhoIβγ (δβγ), or the chimeras listed above. The relative response represents the ratio of the peak benzamil-sensitive current following vertical perfusion to the basal benzamil-sensitive current (Ipeak/Ibasal). C: time constants (τ) of activation by LSS of wild-type and chimeras. Statistically significant differences are indicated as *P < 0.05, **P < 0.01, and ***P < 0.001 (n = 15–29) by a Kruskal-Wallis test following by Dunn's multiple comparisons test.