Fig. 3.

Still images showing renal pelvis of C57BL6/J mice given variable amounts of bubbles. A: fundamental mode of C57BL6/J mouse depicting kidney (kid) and renal pelvis at baseline. B: subharmonic ultrasound mode image following infusion of 100 μl of contrast into the bladder shows no bubbles in the renal pelvis. C: subharmonic ultrasound mode image following 200 μl of bubbles injected into the bladder shows trace amounts of contrast in the pelvis (arrowhead). D: overlay of fundamental and subharmonic modes confirms bubbles (green) within the renal pelvis. Scale bars = 2.5 mm.