Table 2. Design rules for generating systems with desired steady-state or kinetic properties.

Steady-state design	Ultrasensitivity (nH)	Steady-state setpoint (Ss)	Example strain(s)	Relevant figures (see also Table I)
1	Low	Low	cRcTF, sRcTF, tRcTF, cRtTF	Figures 2E and F
2	Low	High	cRcTF with stronger constitutive TF promoter	Supplementary Figure S3
3	High	Low	tRtTF with weaker TF feedback promoter	Supplementary Figure S3
4	High	High	sRtTF, tRtTF	Figure 2F
Kinetic design	Activation (Act50)	Deactivation (Deact50)	Example strain(s)	Relevant figures (see also Table I)
1	Slow	Slow	sRtTF, tRtTF	Figures 2C and D
2	Intermediate	Intermediate	cRtTF	Figures 2C and D
3	Fast	Fast	cRcTF, sRcTF, tRcTF	Figures 2C and D