Table 1. Parameters used in the LA model.
Item | Value or formula | Item | Value or formula |
---|---|---|---|
fq are chosen within the measured range of Tar/Tsr ratio (Kalinin et al, 2010); EqL are chosen to reflect the estimated Kd differences between active and inactive receptors binding with ligand (Mello et al, 2004; Mello and Tu, 2005); αq are from previous experimental measurements and estimates (Shimizu et al, 2010); mq,0 are estimated from our experiments shown in Figure 4C and D, E1,0M is set to be 3.75 to further suppress the activity of Tar for the m=0 state; CJ is designed to be symmetric, meanwhile provide high sensitivity (Mello and Tu, 2003b; Mello et al, 2004); kR(q) and kB(q) are set to be the same for Tar and Tsr receptors for convenience; all the energy parameters are in the unit of kBT, and the timescale is set by having kR(1)=1. | |||
f 1 | 1/2 | f 2 | 1/2 |
E 1 L | 8 | E 2 L | 3 |
m 1,0 | 1 | m 2,0 | 2.5 |
α1 | −1.875 | α2 | −1.0 |
C 1,1 J | −5.5 | C 1,2 J | −6.0 |
C 2,1 J | −6.0 | C 2,2 J | −6.0 |
k R (1) | 1 | k B (1) | 2 |
k R (2) | 1 | k B (2) | 2 |
K I 1 | 18.1 μM | K I 2 | 6.0 μM |