Fig. 1.

Localized ubiquitination (Ub) at the intercalated disc is enhanced by rapamycin treatment during pressure overload (PO). A: Triton-insoluble proteins were normalized to actin and analyzed by immunoblotting with anti-ubiquitin antibody. The summary data for quantification conducted using ImageJ is represented in the graph as means ± SE. *P < 0.05 vs. control; #P < 0.05 vs. PO vehicle. (n = 4). RV, right ventricle; LV, left ventricle; Rapa, rapamycin; Cont, control. B: confocal microscopic analysis was performed on 10-μm sections of PO ventricles from vehicle (top) or rapamycin-treated (bottom) rats stained with anti-N-cadherin (green) and anti-ubiquitin (red). The higher-magnification images below each treatment group show anti-N-cadherin (green) and anti-ubiquitin (red) on the left and anti-α-actinin (green) and anti-ubiquitin (red) on the right. Scale = 10 μm.