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Activation of Notch3 signaling by the exogenous agonist Jagged1 (Jag1)-Fc (1 μg/ml). Cultured VSMCs were exposed to Jag1-Fc (1 μg/ml) for up to 3 h, and the expression levels of NECD, PIC, Hes1, and β-actin were determined by Western blot analysis. Data are expressed as percent changes in protein level from 0 h (normalized to β-actin) and are presented as means ± SE; n = 3. Statistically significant differences from time 0 (*P < 0.05; ***P < 0.001) are indicated.
