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Sphingosine-1-phosphate (S1P) lyase (SPL) expression and activity in the murine heart. A: immunohistochemical detection of a β-galactosidase (β-gal) reporter in hearts of SPL gene-trap reporter mice and reporterless littermate controls. β-Gal was detected in the endothelium (arrows), endocardium lining the valve (V), and cardiomyocytes. B: SPL protein expression in wild-type (WT) heart tissue. See text for details. mSPL, murine SPL.
