Fig. 6.

Dynamics of the inflammatory response in the infarcted region. A: representative images of immunofluorescent staining of heart tissue specimens for the detection of ED-1-positive cells (macrophages) in the infarcted area (the secondary antibody was conjugated with FITC). B: statistical comparisons. C: infarcted region at 14 days after MI, showing representative images of double immunofluorescent staining for the detection of myeloperoxidase (MPO; TRITC labeled) and TUNEL (FITC labeled). Nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI). Arrows indicate TUNEL and MPO double-positive cells. D: dynamics of myofibroblast infiltration in infarct scars from infarcted segment (α-SMA immunofluorescent staining, FITC labeled; nuclei were stained with DAPI). E: MPO immunofluorescent staining (FITC labeled) in the infarcted area at 3 days after MI. F and G: related comparisons from C. H: related comparisons from E. I: related comparisons from D. *P < 0.05 vs. the I-infarct group at the same time point; **P < 0.01 vs. the I-infarct group at the same time point.