Figure 4.

Bone marrow-derived cells are the major producer of IL-1 in inflammation to dead cells. (A) Total neutrophil numbers in peritoneal cavity were determined in bone marrow chimeric mice 14 hours after i.p. challenge of heat-shocked necrotic EL4 cells. Chimeric mice were generated using C57BL/6 (WT) mice as hosts and the indicated IL-1^−/− mice as donors (WT→WT, IL-1α^−/−→WT, IL-1β^−/−→WT, IL-1αβ^−/−→WT), or using IL-1^−/− mice as hosts and IL-1^−/− mice as donors (IL-1α^−/−→IL-1α^−/−, IL-1β^−/−→IL-1β^−/−, IL-1α^−/−β^−/−→IL-1α^−/−β^−/−). (B) Total neutrophil numbers in the peritoneal cavity were determined in bone marrow chimeric mice 14 hours after i.p. challenge of heat-shocked necrotic EL4 cells. Chimeric mice were generated by using IL-1^−/− as hosts and C57BL/6 as donors (WT→ IL-1α^−/−, WT→IL-1β^−/−). (A, B) All of data displays are combined results of 3 or more experiments and represented as means ± SEM (n = total number of mice from the multiple experiments for each group In panel A, One – way ANOVA and Bonferroni's multiple comparison test for all of the groups showed a significant difference for WT→WT to all the other groups (P<0.001), but there was no significant difference among the groups of cells fromIL-1−/− donors (not shown in the figure). In panel B, Bonferroni's multiple comparison test for all of the groups showed a significant difference for WT→ IL-1α^−/− to WT→ IL-1β^−/−, IL-1α^−/− → IL-1α^−/− or IL-1β^−/− → IL-1β^−/− (P<0.01, not shown in the figure). ***p<0.001, **p<0.01, N.S., not significant versus EL4 stimulated WT→WT group.