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. 2011 Feb 22;30(7):1277–1288. doi: 10.1038/emboj.2011.43

Figure 3.

Figure 3

Purified RSC could rescue the nucleosome positioning activity of an extract generated from an rsc3-ts mutant grown under restrictive conditions. DNaseI indirect end labelling analysis of the (A) PHO8, (B) RIM9, (C) CHA1, and (D) SNT1 promoter regions as in Figure 2, but with WCEs generated from wild-type (BY4741) grown logarithmically at 30°C, or from rsc3-ts strain (TH8239) grown logarithmically at 25°C with or without an overnight shift to 37°C. Addition of RSC corresponded to the 1:5 ratio in Figure 2.