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RSC was specifically required for nucleosome positioning in vitro as both SWI/SNF and Isw2 failed to rescue the rsc3-ts 37°C extract. DNaseI indirect end labelling analysis of the (A) PHO8, (B) RIM9, (C) CHA1, and (D) SNT1 promoter regions as in Figures 2 and 3 but with addition of purified SWI/SNF or Isw2 remodelling enzymes as indicated. All remodelling enzymes were added at the same molar concentrations, corresponding to the 1:5 ratio in Figure 2.
