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. 2011 Feb 22;30(7):1251–1262. doi: 10.1038/emboj.2011.40

Figure 1.

Figure 1

(A) Schematic representation of the full-length CaMKII gene and the monomer construct used for these studies. The regulatory domain is highlighted in red with the CaM-binding region in orange. The R1, R2, and R3 refer to segments of the regulatory domain defined previously (Chao et al, 2010). (B) Far-UV and (C) near-UV circular dichroism analysis for the wild-type and cysteine-less CaMKII (WT*) along with their D135N inactivated counterparts. The spectra are almost superimposable confirming the lack of substantial structural changes due to the cysteine substitutions. (D) Stability curves demonstrating the lack of substantial effects on melting temperature by the cysteine substitutions.