Table 3. Relative HPLC peak areas at 280 nm for TβRI:TβRII:TGF-β dimer complexes.
Complex | Relative integrated HPLC peak areasa |
||
---|---|---|---|
TβRI | TβRII | TGF-β dimer | |
Theoretical, 2:2:1 | 0.085 | 0.41 | 1.00 |
Theoretical, 1:1:1 | 0.043 | 0.20 | 1.00 |
HPLC, TGF-β3 WW | 0.099 | 0.45 | 1.00 |
HPLC, TGF-β3 WD | 0.043 | 0.16 | 1.00 |
aIntegrated HPLC peak areas are uncorrected for differences in molar extinction coefficients. |