Fig. 1.

Bile duct ligation (BDL) at 1 and 3 days postsurgery leads to alterations in expression of multiple genes encoding proteins involved in epigenetic modification. Arrow indicates changes in mixed lineage leukemia 3 (MLL3) [a histone H3 lysine 4 (H3K4) methylase] expression. C and D: quantitative (q)PCR analysis of relative expression of nuclear receptor coactivator 6 (NCOA6; C) and MLL4 (D), respectively, during BDL was analyzed separately. qPCR of TNFα and IL-6 mRNA was used as positive controls in C. For the sake of clarity, the values for 1- and 3-day sham samples are not plotted and y-axis shows values relative to the sham controls (arbitrarily set as 1.0) for TNFα, IL-1β, and NCOA6 mRNAs *P value <0.05. #P value not significant.