Fig. 5.

CA3 pyramidal neurons are unaffected in Dlx1 knockout mice. A–D′: no detectable apoptosis is observed in the CA3 pyramidal region of adult control (A–A′ and C–C′) and Dlx1 mutant (B-B′ and D–D') hippocampi as revealed by active caspase-3 antibody (C' and D') and terminal deoxynucleotidyl transferase dUTP-mediated nick-end labeling (A' and B') staining of the corresponding DAPI-labeled (A, B, C, and D) sections. E and F: compared with control (E), no significant change in the number of NeuN⁺ cells was noticed within the CA3 pyramidal layer of the Dlx1 mutant (F) hippocampus. G: quantification of the number of NeuN⁺ cells in the CA3 pyramidal layer of the adult hippocampus. Data shown are means ± SE. Scale bar = 250 μm.