Skip to main content
. 2001 Feb;12(2):265–277. doi: 10.1091/mbc.12.2.265

Figure 6.

Figure 6

Figure 6

Cross talk between Cdc42 and Rac1 is required for substratum-stimulated membrane activity, whereas RhoA activity is inhibitory. (A and B) CHO cells were transfected with control vector, or vectors expressing N17Cdc42 (dominant negative Cdc42), N17Rac1 (dominant negative Rac1), or wild-type RhoA. Analysis was performed using serum-starved cells 2–4 h after plating on an intermediate concentration of fibronectin. (A) Summarized results from three experiments using transient membrane protrusion analysis. The transient protrusion index was determined for the control and standardized to 100. The transient protrusion index for other conditions was determined as a percentage of control with the error bars representing the SD. (B) Histogram showing results from three experiments using stabilized membrane protrusion analysis. The standardized stabilized protrusion was determined for controls and standardized to 100. The relative standardized stabilized protrusion for other conditions was determined as a percentage of control with the error bars representing the SD. (C) Dominant negative Cdc42 inhibits the transient membrane activity stimulated by dominant negative Rac1. Cells were cotransfected in a 1:8 ratio with pIRES-EGFP-N17Rac and either pcDNA3.1 (control) or pEXV-N17Cdc42. Cotransfected cells were identified through fluorescence and transient membrane activity was quantified as described in MATERIALS AND METHODS. The transient protrusion index was determined for the control and standardized to 100. The transient protrusion index for cells cotransfected with N17Cdc42 was determined as a percentage of control with the error bars representing the SD.