Table 1.
Analyses of LLC tumor sections showing effects of different treatments on tumor infiltration by immune cells and apoptosis
| Treatment group | Mean number/HPF ± SEM╪ | |||
|---|---|---|---|---|
| Th cells (CD4) | Cytolytic T cells (CD8) | Apoptotic cells (TUNEL) | Proliferating cells (Ki-67) | |
| AdCMV(-) | 14.8 ± 2.8 | 20.6 ± 4.9 | 2.1 ± 1.2 | 437.4 ± 39.7 |
| AdhTERTHRP | 67.2 ± 7.7 | 79.1 ± 9.4 | 25.8 ± 3.3 | 471.5 ± 51.7 |
| AdCMVmIL-12 | 109.6 ± 11.0 | 143.1 ± 38.7 | 14.4 ± 1.7 | 409.6 ± 36.1 |
| Combination | 139.1 ± 17.6 | 177.2 ± 12.6 | 40.2 ± 4.4 | 463.3 ± 55.4 |
| P* | < 0.0001 | < 0.0001 | < 0.0001 | > 0.05 |
╪After initial scanning under × 100 magnification, positive stained cells in 10 fields under × 400 (0.15 mm2) magnification were counted and the mean number/high-power field (HPF) was determined.
*Statistical analyses were conducted with one-way ANOVA for all the parameters. P values less than 0.05 were considered statistically significant.