Table 1.
Souce, dilution, pretreatment and cutoff values of primary antibodies used
| Antibody (clone) | Manufacturer | Dilution | Pretreatment | Cutoff values |
|---|---|---|---|---|
| Maspin (EAW24) | Novocastra | 1:200 | Microwave | ≧10% (positive) |
| CK5/6 (D5/16B4) | Dako | 1:50 | Water bath | ≧10% (positive) |
| CK14 (LL002) | Novocastra | 1:100 | Water bath | ≧10% (positive) |
| EGFR (EGFR.113) | Novocastra | 1:25 | Microwave | ≧10% (positive) |
| CD10 (56C6) | Novocastra | 1:50 | Water bath | ≧10% (positive) |
| p63 (4A4+Y4A3) | LabVision | 1:200 | None | ≧10% (positive) |
| ER (1D5) | Dako | 1:50 | Water bath | ≧10% (positive) |
| PgR (PgR636) | Dako | 1:800 | Water bath | ≧10% (positive) |
| HER2 | Dako | Prediluted (Hercep test) | Water bath | Score 3+ (positive) |