Table 1.
List of primers used for cloning and quantitative RT-PCR.
| Step | Gene name | Strand | Sequence | Product size (bp) |
|---|---|---|---|---|
| cloning | cx30.9 | Sense | 5'-GAAATTCCGAGGAGGTGGTC-3' | 1,088 |
| Antisense | 5'-GCATGTTTGTCCAGGACTAC-3' | |||
| cloning | cx34.3 | Sense | 5'-CTGAGAGACTGAGAGCTCCAAAC-3' | 1,038 |
| Antisense | 5'-AGACTGGCATGCGAATCATTG-3' | |||
| cloning | cx43.2 | Sense | 5'-TCAAGAAGTGACGGAGAAAG-3' | 1,278 |
| Antisense | 5'-GTACTCTCTGTTCTCTGGCA-3' | |||
| cloning | cx44.9 | Sense | 5'-AGCCTTAAAGAGCTGAGAGG-3' | 1,273 |
| Antisense | 5'-CGAGCAGGCATTAAGCAGGA-3' | |||
| qPCR* | cx30.9 | Antisense | 5'-GGCTGGTGGAGTGTTTGTTC-3' | 99 |
| qPCR | cx34.3 | Sense | 5'-ACTACCTGTATGGCTTCACCCT-3' | 284 |
| Antisense | 5'-CTGGATCATCTGGTCTTTGTTC-3' | |||
| qPCR | cx43.2 | Sense | 5'-ATGGCTGTTCCTCTCCCACT-3' | 121 |
| Antisense | 5'-CAGTTTTGCTCGTTGGCTTG-3' | |||
| qPCR | cx44.9 | Sense | 5'-GTGACAGAAGGCTCTGTGAAG-3' | 305 |
| Antisense | 5'-ACACCGAGATGGAGAATCTC-3' |
*qPCR for cx30.9 was performed using cx30.9 cloning primer with the antisense qPCR primer for cx30.9.