Figure 3.

PlexinB knockdown decreases axonal complexity. A, Wildtype flies have a stereotyped pSc axonal branching pattern. B, PlexB^LOF/+ do not have axon guidance errors and have wildtype branching patterns. C, Reduced PlexinB expression in pSc neurons results in fewer branches, and an overall decrease in arbor growth. Arrows denote missing stereotypical skeleton branches of the pSc arbor that normally occur in wildtype flies. D, Combining PlexinB RNAi with PlexB^LOF/+ phenocopies the 455-Gal4; UAS-PlexB-RNAi pSc axons, and does not induce axon guidance errors. Dotted lines represent the midline and the scale bar is 50 μm.