Table 1.
Primer sets used in this study
| Primer | Sequence (5'→ 3') | Polarity | Regiona | |
|---|---|---|---|---|
| Diagnosis primer sets | GIF1M | CTG CCC GAA TTY GTA AAT GAT GAT | + | 5342-5365 |
| GIRIM | CCA ACC CAR CCA TTR TAC ATY TG | - | 5649-5671 | |
| GIIF1M | GGG AGG GCG ATC GCA ATC T | + | 5049-5067 | |
| GIIRIM | CCR CCI GCA TRI CCR TTR TAC AT | - | 5367-5389 | |
| Designed primer sets | ORF1-1F | GTG AAT GAA GAT GGC GTC TAA C | + | 1-22 |
| ORF1-1R | AGT TCC ACT GCA AGG TCC TCA G | - | 999-1020 | |
| ORF1-2F | CTG AGG ACC TTG CAG TGG AAC T | + | 999-1020 | |
| ORF1-2R | ATG AGG GAA CCA GTG GTG AGA GT | - | 2018-2040 | |
| ORF1-3F | CGT GCT CGA GGC GCA TCG ATT T | + | 1821-1842 | |
| ORF1-3R | TTG TAC TCA TCA TAC TCT TCA | - | 2701-2721 | |
| ORF1-4F | CAT TGC TCG AGC ATC AGG GCT AC | + | 2038-2060 | |
| ORF1-4R | TTG ACT ATC CTC GAC CAG ATG CT | - | 3038-3060 | |
| ORF1-5F | AGC ATC TGG TCG AGG ATA GTC AA | + | 3038-3060 | |
| ORF1-5R | GTG GCA CAT ATG ACA GTG TTT CC | - | 3518-3540 | |
| ORF1-6F | GGA AAC ACT GTC ATA TGT GCC AC | + | 3518-3540 | |
| ORF1-6R | CAG TCG TTC TTC CGC ATG TGG TGC GG | - | 3935-3960 | |
| ORF1-7F | CTC AGC ACC AAG ACT AAA TTC TGG A | + | 3635-3659 | |
| ORF1-7R | TGG GCG ATG GAA TTC CAT TGA GAG G | - | 4485-4509 | |
| ORF1-8F | ATG GTT AAA TTC TCC CCA GAA CC | + | 4355-4377 | |
| ORF1-8R | CCA CCT GCA TAA CCA TTG TAC AT | - | 5367-5389 | |
| ORF2-F | ATG AAG ATG GCG TCG AAT GGC | + | 5085-5105 | |
| ORF2-R | TTA TAA TGC ACG CCT GCG CCC CGT | - | 6681-6704 | |
| ORF3-F | ATG GCT GGA GCT TTC TTT GCT | + | 6704-6724 | |
| ORF3-R | AAA GAC ACT AAA GAA AGG AAA GAT | - | 7532-7555 | |