Table 1.
Primers | Position | Sequence(5'-3') | Fragment |
---|---|---|---|
S1 b | Used for 5'-RACE | CATGGCTACATGCTGACAGCCTA | 5'RACE |
S2 | 344-367 | TGAAAGGCGGGCGCTTGGTGACA | 5'RACE |
U1 | 379-399 | TAAgTTTTACCGTCGTTCCC | UFR |
U2 | 718-736 | ACCGAGCGTGGAGTCAAT | UFR |
A1 | 524-541 | CGGAAGTAAAACGGCACA | A |
A2 | 2276-2293 | GATTTCCAACAGCGGTCA | A |
B1 | 2217-2233 | CGTGTTTGGCAGCCTCAT | B |
B2 | 4700-4717 | CCACGGGTTCAGGTCTCG | B |
C1 | 4576-4593 | AACGGCCCAAGCAAGTAT | C |
C2 | 6599-6616 | TGGAAACGCACGAGCAGT | C |
D1 | 6205-6222 | GGCAGAGCCATGACAGAC | D |
D2 | 7458-7475 | GGGTGGAAGCCAAACTCT | D |
GSP1 | 7446-7463 | GGTGTTTCGCACAGAGTT | 3'RACE |
R1 | 3'end | TAAgCAAgCATgCCATATgTT | 3'RACE |
3' RACERTc | Used for 3'-RACE | TAAgCAAgCATgCCATATg(T)15 |
a The primers used in this study were designed based on JS/CHA/05 (Accession NO. EF149009)
b S1 was used in 5'-RACE for amplification of the 5' end of RNA.
c 3'RACERT was used in 3'-RACE for amplification of the 3' end of RNA.