Figure 10.
Aggregation of MDCK and MSV-MDCK cells expressing E-cadherin and β-subunit of Na,K-ATPase. Single cells (200,000) were plated in regular culture medium in a plastic Petri dish and incubated at 37°C on a gyratory shaker (80 rpm) for 3 h. Note MSV-Cad vector cells aggregated similar to MDCKwt and MSV-Cad-NaKβ cells. MSV-Cad vector cells were incubated in calcium free medium containing 2.5 mM EDTA to test the calcium dependence of cell aggregation in these cells. Note in the absence of calcium, the aggregation of MSV-Cad vector cells is inhibited by more than 85% compared with cells incubated in the presence of calcium. The aggregation index was calculated as described in MATERIALS AND METHODS. SE bars indicate the average of three independent experiments done in triplicates.