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Immunization protocol of rabbits
| Immunization times | Group A (No.1, No.2) | Group B (No.3, No.4) | Group C (No.5, No.6) | |||
|---|---|---|---|---|---|---|
| Adjuvant | dose(μg/rabbit) | Adjuvant | dose(μg/rabbit) | Adjuvant | dose(μg/rabbit) | |
| 1st | FCA | 350 | ISA 201 | 350 | ISA 206 | 350 |
| 2nd | FIA | 350 | ISA 201 | 350 | ISA 206 | 350 |
| 3rd | None | 350 | None | 350 | None | 350 |
| 4th | None | 350 | None | 350 | None | 350 |
Note: FCA- Freund's complete adjuvant, FIA- Freund's incomplete adjuvant, ISA 201and ISA206 oil adjuvants were purchased from Seppic Company, France.
