Table 2.

The OD_{450 nm} value of block titration

Dilution of sheep-anti-rabbit IgG-HRP	Rabbit sera	Antigen dilution 1:200	Antigen dilution 1:400	Antigen dilution 1:800	Antigen dilution 1:1600	Antigen dilution 1:3200	Blank control
1:10000	positive	3.374	3.279	3.202	2.665	2.010	0.021
	negative	0.076	0.087	0.065	0.076	0.055	0.033
1:20000	positive	2.827	2.691	2.444	2.174	1.891	0.032
	negative	0.066	0.067	0.065	0.045	0.076	0.053
1:40000	positive	2.525	2.398	1.891	1.610	1.472	0.034
	negative	0.062	0.054	0.042	0.031	0.030	0.042
1:80000	positive	1.667	1.407	0.990	0.632	0.415	0.035
	negative	0.045	0.034	0.031	0.029	0.026	0.010