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. 2011 May 13;6(5):e19678. doi: 10.1371/journal.pone.0019678

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Rousseau et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Purified respiratory mucins were reduced and carboxymethylated and dialysed into 6 M urea prior to anion exchange chromatography on a Resource Q column. Aliquots of each fraction were analysed by PAS-staining (black triangles) and for reactivity with MANeq5b-I (black circles) and MANeq5ac-I (open circles). The dotted line indicates the linear gradient of lithium perchlorate. Inset shows Muc5ac and Muc5b peptides identified by tandem mass spectrometry analysis of fractions 20 and 29.