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. 2011 May 13;6(5):e19883. doi: 10.1371/journal.pone.0019883

Figure 4. Schematic representation of plant transformation vector.

Figure 4

(A) Schematic structures of plant transformation vector pDES200-GUS-GFP-IFS-BADH. The T-DNA with left and right borders (LB and RB) is indicated. The different expression units of IFS cassette, BADH cassette, GUS cassette and GFP cassette, including the HPTII cassette, are indicated by orange arrows. (B) The inducible marker-free multigene transformation vector pDES100-GUS-BAR-HPT-free contains two interested genes of gus and bar. The hptII gene is responsible for hygromycin resistance needed for transgenic event selection and flanked by two direct repeat loxP sites, which could be excised by the induction of estrogen. (C) Restriction analysis of recombinant constructs containing different numbers of expression cassettes with restriction enzymes PacI and AscI. The restriction enzymes sites (PacI and AscI) for identification of the vector are also indicated. P1–P4, primers used for PCR analysis. LB: left border; RB: right border. Marker, 1-kb DNA ladder.