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. 2011 May 13;6(5):e19883. doi: 10.1371/journal.pone.0019883

Figure 6. Identification of Cre/loxP-mediated DNA recombination event.

Figure 6

(A) PCR analysis of genomic DNA prepared from uninduced T1 plant and recombinant T2 transgenic plants (lines1, 2 and 3) using primers P1/P2, P3/P4, and P1/P4. Primer pairs used for each PCR reaction are indicated on the top of each lane. The expected PCR products from different combinations of primer pairs are indicated on the right. No amplification was observed with the genomic DNA of wild-type plants (WT) using any of the three primer pairs. M, 1-kb DNA ladder. (B) Sequence confirmation of marker gene excision in the genome of transgenic T2 lines. Part of the sequence at excision site is exactly as predicted, indicating that the excision occurred and all DNA sequences, including hptII gene, between two loxP sites were excised, with only one intact loxP site left.